CRISPR/Cas9 Prime Editing is a novel gene editing technique that utilizes a modified Cas9 protein to introduce mutations in the genome with a higher degree of safety and complexity. This technique represents an evolution in the CRISPR/Cas9 system that enables researchers to more safely create complex genetic changes than using previous genome editing methods. In this article, we will explore the mechanisms of CRISPR/Cas9 Prime Editing, its advantages and disadvantages over other gene editing methods, and its potential applications in biotechnology and medicine. Mechanisms of CRISPR/Cas9 Prime Editing: CRISPR/Cas9 Prime Editing employs two main components to drive its function. The first